5-HT1B receptor-mediated presynaptic inhibition of GABA release in the suprachiasmatic nucleus

Jayne R. Bramley, Patricia J. Sollars, Gary E. Pickard, F. Edward Dudek

Research output: Contribution to journalArticle

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Abstract

The suprachiasmatic nucleus (SCN) receives a dense serotonergic innervation that modulates photic input to the SCN via serotonin 1B (5-HT1B) presynaptic receptors on retinal glutamatergic terminals. However, the majority of 5-HT1B binding sites in the SCN are located on nonretinal terminals and most axonal terminals in the SCN are GABAergic. We therefore tested the hypothesis that 5-HT1B receptors might also be located on SCN GABAergic terminals by examining the effects of the highly selective 5-HT1B receptor agonist CP-93,129 on SCN miniature inhibitory postsynaptic currents (mIPSCs). Whole cell patch-clamp recordings of mIPSCs were obtained from rat and mouse SCN neurons in hypothalamic slices. Using CsCl-containing microelectrodes with QX314, we isolated mPSCs that were sensitive to the GABAA receptor antagonist, bicuculline. Bath application of CP-93,129 (1 μM) decreased the frequency of mIPSCs by an average of 22% (n = 7) in rat SCN neurons and by an average of 30% (n = 8) in mouse SCN neurons with no clear effect on mIPSC amplitude. In mice lacking functional 5-HT1B receptors, CP-93, 129 (1 μM) had no clear effect on the frequency or the amplitude of mIPSCs recorded in any of the cells tested (n = 4). The decrease in the frequency of mIPSCs of SCN neurons produced by the selective 5-HT1B receptor agonist CP-93,129 is consistent with the interpretation that 5-HT1B receptors are located on GABA terminals in the SCN and that 5-HT inhibits GABA release via a 5-HT1B presynaptic receptor-mediated mechanism.

Original languageEnglish (US)
Pages (from-to)3157-3164
Number of pages8
JournalJournal of Neurophysiology
Volume93
Issue number6
DOIs
StatePublished - Jun 1 2005

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Receptor, Serotonin, 5-HT1B
Suprachiasmatic Nucleus
gamma-Aminobutyric Acid
Inhibitory Postsynaptic Potentials
Serotonin 5-HT1 Receptor Agonists
Presynaptic Receptors
Neurons
Serotonin
GABA-A Receptor Antagonists
Bicuculline
Microelectrodes
Baths

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

5-HT1B receptor-mediated presynaptic inhibition of GABA release in the suprachiasmatic nucleus. / Bramley, Jayne R.; Sollars, Patricia J.; Pickard, Gary E.; Dudek, F. Edward.

In: Journal of Neurophysiology, Vol. 93, No. 6, 01.06.2005, p. 3157-3164.

Research output: Contribution to journalArticle

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