3-Methylcholanthrene-inducible binding of aromatic hydrocarbons to DNA in purified rat liver nuclei

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Abstract

Purified rat liver nuclei contain enzymes capable of activating polycyclic aromatic hydrocarbons which can then bind to nuclear DNA. This has been demonstrated by incubating nuclei with [14C] hydrocarbon and purifying the nuclear DNA containing bound hydrocarbon. Intraperitoneal injection of the rats with 3-methylcholanthrene twenty-four hours before livers were removed raised the level of hydrocarbon binding to nuclear DNA four-fold. Omission of reduced nicotinamide adenine dinucleotide phosphate from incubation mixtures caused a four-fold decrease in the ability of 3-methylcholanthrene induced nuclei to bind hydrocarbon to nuclear DNA. The most potent carcinogens in a series of seven hydrocarbons displayed a relatively higher extent of binding to nuclear DNA. The data suggest that nuclear aryl hydroxylases effect covalent binding of hydrocarbons to DNA and presumably initiate the cancer process.

Original languageEnglish (US)
Pages (from-to)1119-1126
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume58
Issue number4
DOIs
StatePublished - Jun 18 1974

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Aromatic Hydrocarbons
Methylcholanthrene
Hydrocarbons
Liver
Rats
DNA
Polycyclic Aromatic Hydrocarbons
Mixed Function Oxygenases
Intraperitoneal Injections
NADP
Carcinogens
Enzymes
Neoplasms

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "3-Methylcholanthrene-inducible binding of aromatic hydrocarbons to DNA in purified rat liver nuclei",
abstract = "Purified rat liver nuclei contain enzymes capable of activating polycyclic aromatic hydrocarbons which can then bind to nuclear DNA. This has been demonstrated by incubating nuclei with [14C] hydrocarbon and purifying the nuclear DNA containing bound hydrocarbon. Intraperitoneal injection of the rats with 3-methylcholanthrene twenty-four hours before livers were removed raised the level of hydrocarbon binding to nuclear DNA four-fold. Omission of reduced nicotinamide adenine dinucleotide phosphate from incubation mixtures caused a four-fold decrease in the ability of 3-methylcholanthrene induced nuclei to bind hydrocarbon to nuclear DNA. The most potent carcinogens in a series of seven hydrocarbons displayed a relatively higher extent of binding to nuclear DNA. The data suggest that nuclear aryl hydroxylases effect covalent binding of hydrocarbons to DNA and presumably initiate the cancer process.",
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T1 - 3-Methylcholanthrene-inducible binding of aromatic hydrocarbons to DNA in purified rat liver nuclei

AU - Rogan, Eleanor G

AU - Cavalieri, Ercole

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N2 - Purified rat liver nuclei contain enzymes capable of activating polycyclic aromatic hydrocarbons which can then bind to nuclear DNA. This has been demonstrated by incubating nuclei with [14C] hydrocarbon and purifying the nuclear DNA containing bound hydrocarbon. Intraperitoneal injection of the rats with 3-methylcholanthrene twenty-four hours before livers were removed raised the level of hydrocarbon binding to nuclear DNA four-fold. Omission of reduced nicotinamide adenine dinucleotide phosphate from incubation mixtures caused a four-fold decrease in the ability of 3-methylcholanthrene induced nuclei to bind hydrocarbon to nuclear DNA. The most potent carcinogens in a series of seven hydrocarbons displayed a relatively higher extent of binding to nuclear DNA. The data suggest that nuclear aryl hydroxylases effect covalent binding of hydrocarbons to DNA and presumably initiate the cancer process.

AB - Purified rat liver nuclei contain enzymes capable of activating polycyclic aromatic hydrocarbons which can then bind to nuclear DNA. This has been demonstrated by incubating nuclei with [14C] hydrocarbon and purifying the nuclear DNA containing bound hydrocarbon. Intraperitoneal injection of the rats with 3-methylcholanthrene twenty-four hours before livers were removed raised the level of hydrocarbon binding to nuclear DNA four-fold. Omission of reduced nicotinamide adenine dinucleotide phosphate from incubation mixtures caused a four-fold decrease in the ability of 3-methylcholanthrene induced nuclei to bind hydrocarbon to nuclear DNA. The most potent carcinogens in a series of seven hydrocarbons displayed a relatively higher extent of binding to nuclear DNA. The data suggest that nuclear aryl hydroxylases effect covalent binding of hydrocarbons to DNA and presumably initiate the cancer process.

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