2.45 GHz radiofrequency fields alter gene expression in cultured human cells

Sanggyu Lee, Debra Johnson, K. Dunbar, Hui Dong, Xijin Ge, Yeong C. Kim, Claudia Wing, Nimanthi Jayathilaka, Nimmi Emmanuel, Chenn Q. Zhou, Howard L. Gerber, Charles C. Tseng, San Ming Wang

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The biological effect of radiofrequency (RF) fields remains controversial. We address this issue by examining whether RF fields can cause changes in gene expression. We used the pulsed RF fields at a frequency of 2.45 GHz that is commonly used in telecommunication to expose cultured human HL-60 cells. We used the serial analysis of gene expression (SAGE) method to measure the RF effect on gene expression at the genome level. We observed that 221 genes altered their expression after a 2-h exposure. The number of affected genes increased to 759 after a 6-h exposure. Functional classification of the affected genes reveals that apoptosis-related genes were among the upregulated ones and the cell cycle genes among the downregulated ones. We observed no significant increase in the expression of heat shock genes. These results indicate that the RF fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism.

Original languageEnglish (US)
Pages (from-to)4829-4836
Number of pages8
JournalFEBS Letters
Volume579
Issue number21
DOIs
StatePublished - Aug 29 2005

Fingerprint

Gene expression
Cultured Cells
Genes
Cells
Gene Expression
Telecommunications
cdc Genes
HL-60 Cells
Shock
Down-Regulation
Hot Temperature
Genome
Apoptosis
Telecommunication

Keywords

  • Biological effect
  • Gene expression
  • Radiofrequency
  • SAGE

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Lee, S., Johnson, D., Dunbar, K., Dong, H., Ge, X., Kim, Y. C., ... Wang, S. M. (2005). 2.45 GHz radiofrequency fields alter gene expression in cultured human cells. FEBS Letters, 579(21), 4829-4836. https://doi.org/10.1016/j.febslet.2005.07.063

2.45 GHz radiofrequency fields alter gene expression in cultured human cells. / Lee, Sanggyu; Johnson, Debra; Dunbar, K.; Dong, Hui; Ge, Xijin; Kim, Yeong C.; Wing, Claudia; Jayathilaka, Nimanthi; Emmanuel, Nimmi; Zhou, Chenn Q.; Gerber, Howard L.; Tseng, Charles C.; Wang, San Ming.

In: FEBS Letters, Vol. 579, No. 21, 29.08.2005, p. 4829-4836.

Research output: Contribution to journalArticle

Lee, S, Johnson, D, Dunbar, K, Dong, H, Ge, X, Kim, YC, Wing, C, Jayathilaka, N, Emmanuel, N, Zhou, CQ, Gerber, HL, Tseng, CC & Wang, SM 2005, '2.45 GHz radiofrequency fields alter gene expression in cultured human cells', FEBS Letters, vol. 579, no. 21, pp. 4829-4836. https://doi.org/10.1016/j.febslet.2005.07.063
Lee S, Johnson D, Dunbar K, Dong H, Ge X, Kim YC et al. 2.45 GHz radiofrequency fields alter gene expression in cultured human cells. FEBS Letters. 2005 Aug 29;579(21):4829-4836. https://doi.org/10.1016/j.febslet.2005.07.063
Lee, Sanggyu ; Johnson, Debra ; Dunbar, K. ; Dong, Hui ; Ge, Xijin ; Kim, Yeong C. ; Wing, Claudia ; Jayathilaka, Nimanthi ; Emmanuel, Nimmi ; Zhou, Chenn Q. ; Gerber, Howard L. ; Tseng, Charles C. ; Wang, San Ming. / 2.45 GHz radiofrequency fields alter gene expression in cultured human cells. In: FEBS Letters. 2005 ; Vol. 579, No. 21. pp. 4829-4836.
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