1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

Jiang Jiang, Yang Zhang, Arup K. Indra, Gitali Ganguli-Indra, Mai N. Le, Hongjun Wang, Ronald R. Hollins, Debra A. Reilly, Mark A. Carlson, Richard L. Gallo, Adrian F. Gombart, Jingwei Xie

Research output: Contribution to journalArticle

Abstract

Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

LanguageEnglish (US)
Pages1417-1432
Number of pages16
JournalNanomedicine
Volume13
Issue number12
DOIs
StatePublished - Jun 1 2018

Fingerprint

Nanofibers
immunity
Bandages
induction
peptide
Peptides
skin
efficiency
encapsulation
Skin
Wounds and Injuries
U937 Cells
Calcitriol
Electrospinning
Keratinocytes
Encapsulation
Innate Immunity
Transgenic Mice
Monocytes
dihydroxy-vitamin D3

Keywords

  • 1α25-dihydroxyvitamin D
  • antimicrobial peptides LL-37
  • electrospun nanofibers
  • endogenous production
  • innate immunity
  • sustained delivery

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Development

Cite this

1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression. / Jiang, Jiang; Zhang, Yang; Indra, Arup K.; Ganguli-Indra, Gitali; Le, Mai N.; Wang, Hongjun; Hollins, Ronald R.; Reilly, Debra A.; Carlson, Mark A.; Gallo, Richard L.; Gombart, Adrian F.; Xie, Jingwei.

In: Nanomedicine, Vol. 13, No. 12, 01.06.2018, p. 1417-1432.

Research output: Contribution to journalArticle

Jiang, Jiang ; Zhang, Yang ; Indra, Arup K. ; Ganguli-Indra, Gitali ; Le, Mai N. ; Wang, Hongjun ; Hollins, Ronald R. ; Reilly, Debra A. ; Carlson, Mark A. ; Gallo, Richard L. ; Gombart, Adrian F. ; Xie, Jingwei. / 1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression. In: Nanomedicine. 2018 ; Vol. 13, No. 12. pp. 1417-1432.
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