γ-aminobutyric acid plasma levels and brain binding in Eck fistula dogs

Jon S. Thompson, Daniel F. Schafer, Gregory J. Schafer, Paul E. Hodgson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

It has been hypothesized that γ-aminobutyric acid (GABA), the principle inhibitory neurotransmitter of the mammalian brain, contributes to the neural inhibition of hepatic encephalopathy. Eck fistulae were created in seven dogs and celiotomy alone performed in five dogs to determine plasma GABA levels, brain GABA binding, and synaptic membrane changes in dogs after creation of Eck fistulae. Eck fistula dogs lost 19 ± 9% body weight, lost hair, ate poorly, and developed atrophic livers with classic hepatic histological changes. Control dogs maintained body weight, normal behavior, and normal liver histology. Plasma GABA levels were elevated significantly in the Eck fistula dogs (312 ± 105.9 nM) both as compared to controls (154.4 ± 69.8) and to preoperative levels (161.6 ± 56.7, P < 0.05). Brain GABA binding for animals sacrificed at 6-9 weeks was not statistically different from sham-operated dogs sacrificed at 6 weeks (1.87 ± .54 pmole/mg protein vs 1.186 ± 24, P < 0.2). Synaptic membrane fluidity and cholesterol were likewise unchanged. Plasma GABA levels are increased significantly following complete portal diversion but do not correlate with the degree of encephalopathy. GABA binding to neural membranes are not significantly increased in Eck fistula dogs. These findings do not support a direct relationship between plasma GABA levels and neurologic impairment in Eck fistula dogs.

Original languageEnglish (US)
Pages (from-to)143-148
Number of pages6
JournalJournal of Surgical Research
Volume38
Issue number2
DOIs
StatePublished - Feb 1985

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Surgical Portacaval Shunt
Aminobutyrates
gamma-Aminobutyric Acid
Dogs
Brain
Synaptic Membranes
Liver
Neural Inhibition
Ideal Body Weight
Membrane Fluidity
Hepatic Encephalopathy
Brain Diseases
Hair
Nervous System
Neurotransmitter Agents
Histology
Cholesterol
Body Weight

ASJC Scopus subject areas

  • Surgery

Cite this

γ-aminobutyric acid plasma levels and brain binding in Eck fistula dogs. / Thompson, Jon S.; Schafer, Daniel F.; Schafer, Gregory J.; Hodgson, Paul E.

In: Journal of Surgical Research, Vol. 38, No. 2, 02.1985, p. 143-148.

Research output: Contribution to journalArticle

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