β1 integrin regulates MMP-10 dependant tubulogenesis in human lymphatic endothelial cells

Maria M. Steele, Amanda M. Schieler, Philip M. Kelley, Richard M. Tempero

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Lymphatic vessel growth requires extensive remodeling of the extracellular matrix, a process hypothesized to be related to the expression and function of the matrix metalloproteinases. We used a protein based screening strategy to demonstrate increased matrix matalloproteinase-10 expression in human lymphatic endothelial cells undergoing collagen I induced tubulogenesis. Knock-down experiments showed that matrix metalloproteinase-10 regulated lymphatic endothelial cell tubulogenesis. β1 integrin signaling via the ERK/MAPK pathway increased matrix metalloproteinase-10 mRNA and protein expression in human lymphatic endothelial cells. These findings demonstrate a novel mechanism by which β1 integrin regulates matrix metalloproteinase-10 expression during lymphatic vessel remodeling.

Original languageEnglish (US)
Pages (from-to)218-224
Number of pages7
JournalMatrix Biology
Volume30
Issue number3
DOIs
StatePublished - Apr 1 2011

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Keywords

  • Endothelial
  • Lymphangiogenesis
  • MMP-10

ASJC Scopus subject areas

  • Molecular Biology

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