β-Hexosaminidase lsozymes in Human Colonic Carcinoma

Michael G. Brattain, Pamela M. Kimball, Thomas G. Pretlow

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Abstract

lsoelectric focusing of crude extracts demonstrated that human colonic carcinomas contained a higher proportion of β-hexosaminidase B than β-hexosaminidase A, while normal human colonic mucosa contained a higher proportion of the A form of the enzyme. Studies of the isolated isozymes showed that β-hexosaminidase B was more stable to heat and more active at low pH than β-hexosaminidase A. Kinetic studies revealed that the A and B forms of β-hexosaminidase had essentially the same Kmand Vmaxfor ρ-nitro-phenyl-N-acetyl-β-D-glucosaminide.

Original languageEnglish (US)
Pages (from-to)731-735
Number of pages5
JournalCancer Research
Volume37
Issue number3
Publication statusPublished - Mar 1977

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Brattain, M. G., Kimball, P. M., & Pretlow, T. G. (1977). β-Hexosaminidase lsozymes in Human Colonic Carcinoma. Cancer Research, 37(3), 731-735.