β-Hexosaminidase lsozymes in Human Colonic Carcinoma

Michael G Brattain, Pamela M. Kimball, Thomas G. Pretlow

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

lsoelectric focusing of crude extracts demonstrated that human colonic carcinomas contained a higher proportion of β-hexosaminidase B than β-hexosaminidase A, while normal human colonic mucosa contained a higher proportion of the A form of the enzyme. Studies of the isolated isozymes showed that β-hexosaminidase B was more stable to heat and more active at low pH than β-hexosaminidase A. Kinetic studies revealed that the A and B forms of β-hexosaminidase had essentially the same Kmand Vmaxfor ρ-nitro-phenyl-N-acetyl-β-D-glucosaminide.

Original languageEnglish (US)
Pages (from-to)731-735
Number of pages5
JournalCancer Research
Volume37
Issue number3
StatePublished - Jan 1 1977

Fingerprint

Hexosaminidase B
Hexosaminidases
Hexosaminidase A
Carcinoma
Complex Mixtures
Isoenzymes
Mucous Membrane
Hot Temperature
Enzymes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Brattain, M. G., Kimball, P. M., & Pretlow, T. G. (1977). β-Hexosaminidase lsozymes in Human Colonic Carcinoma. Cancer Research, 37(3), 731-735.

β-Hexosaminidase lsozymes in Human Colonic Carcinoma. / Brattain, Michael G; Kimball, Pamela M.; Pretlow, Thomas G.

In: Cancer Research, Vol. 37, No. 3, 01.01.1977, p. 731-735.

Research output: Contribution to journalArticle

Brattain, MG, Kimball, PM & Pretlow, TG 1977, 'β-Hexosaminidase lsozymes in Human Colonic Carcinoma', Cancer Research, vol. 37, no. 3, pp. 731-735.
Brattain MG, Kimball PM, Pretlow TG. β-Hexosaminidase lsozymes in Human Colonic Carcinoma. Cancer Research. 1977 Jan 1;37(3):731-735.
Brattain, Michael G ; Kimball, Pamela M. ; Pretlow, Thomas G. / β-Hexosaminidase lsozymes in Human Colonic Carcinoma. In: Cancer Research. 1977 ; Vol. 37, No. 3. pp. 731-735.
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