β-Hairpin stabilization in a 28-residue peptide derived from the β- subunit sequence of human chorionic gonadotropin hormone

R. A G D Silva, Simon Sherman, Timothy A. Keiderling

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The β-subunit of the human chorionic gonadotropin (hCG) hormone, which is believed to be related to certain types of cancer, contains three hairpin- like fragments. To investigate the role of β-hairpin formation in the early stages of the hCGβ folding, a 28-residue peptide with the sequence RDVRFESIRLPGSPRGVNPVVSYAVALS, corresponding to the H3-β hairpin fragment (residues 60-87) of the hCGβ subunit, was studied under various conditions using three optical spectroscopic methods: Fourier transform in spectroscopy, electronic CD, and vibrational CD. Environmental conditions are critical factors for formation of secondary structure in this peptide. TFE : H2O mixed solvents induced helical formation. Formation of β-structure in this peptide, which may be related to the native β-hairpin formation in the intact hormone, was found to be induced only under conditions such as high concentration, high temperature, and the presence of nonmicellar sodium dodecyl sulfate concentrations. These findings support a protein folding mechanism for the hCGβ subunit in which an initial hydrophobic collapse, which increases intermolecular interactions in hCGβ, is needed to induce the H3-β hairpin formation.

Original languageEnglish (US)
Pages (from-to)413-423
Number of pages11
JournalBiopolymers
Volume50
Issue number4
DOIs
StatePublished - Oct 5 1999

Fingerprint

Hormones
Chorionic Gonadotropin
Peptides
Stabilization
Protein folding
Protein Folding
Polytetrafluoroethylene
Sodium dodecyl sulfate
Fourier Analysis
Sodium Dodecyl Sulfate
Spectrum Analysis
Fourier transforms
Gonadotropins
Spectroscopy
Temperature
Neoplasms

Keywords

  • CD
  • Ir spectroscopy
  • Peptide conformation
  • Protein folding
  • β-hairpin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

Cite this

β-Hairpin stabilization in a 28-residue peptide derived from the β- subunit sequence of human chorionic gonadotropin hormone. / Silva, R. A G D; Sherman, Simon; Keiderling, Timothy A.

In: Biopolymers, Vol. 50, No. 4, 05.10.1999, p. 413-423.

Research output: Contribution to journalArticle

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