β-Carotene and α-tocopherol inhibit the development of atherosclerotic lesions in hypercholesterolemic rabbits

J. Sun, D. W. Giraud, Rodney A Moxley, J. A. Driskell

Research output: Contribution to journalArticle

Abstract

Male New Zealand rabbits were made hypercholerolemic by feeding an atherogenic diet (0.5% cholesterol, 3% peanut oil, and 3% coconut oil) with or without antioxidants for 8 weeks. The treatments were IV injection of β-carotene (25 mg/kg BW, twice weekly), dietary α-tocopherol (0.5%), a combination of both, and no added antioxidants (control), β-carotene treatment significantly decreased total and LDL cholesterol concentrations, thoracic atherosclerotic lesion area, and aortic intimai thickness, but had no effects on LDL oxidation ex vivo as compared to control. Dietary a-tocopherol supplementation significantly decreased the susceptibility of LDL to oxidation ex vivo, total atherosclerotic lesion area, and aortic intimai thickness as compared to control. Combination of both antioxidants significantly decreased the susceptibility of LDL to oxidation ex vivo, total atherosclerotic lesion area, and aortic intimai thickness as compared to control, but not β-carotene or α-tocopherol groups. These data suggest that the inhibition of development of atherosclerotic lesions by antioxidants may result from a combination of both antioxidant and nonantioxidant activities. (Supported in part by the Nebraska Agricultural Research Division and Applied Carotenoid Sciences, Inc.).

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

Fingerprint

Tocopherols
Carotenoids
carotenes
tocopherols
lesions (animal)
Antioxidants
rabbits
Rabbits
antioxidants
oxidation
Oxidation
Atherogenic Diet
peanut oil
coconut oil
chest
Nutrition
agricultural research
low density lipoprotein cholesterol
intravenous injection
LDL Cholesterol

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

β-Carotene and α-tocopherol inhibit the development of atherosclerotic lesions in hypercholesterolemic rabbits. / Sun, J.; Giraud, D. W.; Moxley, Rodney A; Driskell, J. A.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

@article{0bee00d3e6da4b46ae2e478cea54d90c,
title = "β-Carotene and α-tocopherol inhibit the development of atherosclerotic lesions in hypercholesterolemic rabbits",
abstract = "Male New Zealand rabbits were made hypercholerolemic by feeding an atherogenic diet (0.5{\%} cholesterol, 3{\%} peanut oil, and 3{\%} coconut oil) with or without antioxidants for 8 weeks. The treatments were IV injection of β-carotene (25 mg/kg BW, twice weekly), dietary α-tocopherol (0.5{\%}), a combination of both, and no added antioxidants (control), β-carotene treatment significantly decreased total and LDL cholesterol concentrations, thoracic atherosclerotic lesion area, and aortic intimai thickness, but had no effects on LDL oxidation ex vivo as compared to control. Dietary a-tocopherol supplementation significantly decreased the susceptibility of LDL to oxidation ex vivo, total atherosclerotic lesion area, and aortic intimai thickness as compared to control. Combination of both antioxidants significantly decreased the susceptibility of LDL to oxidation ex vivo, total atherosclerotic lesion area, and aortic intimai thickness as compared to control, but not β-carotene or α-tocopherol groups. These data suggest that the inhibition of development of atherosclerotic lesions by antioxidants may result from a combination of both antioxidant and nonantioxidant activities. (Supported in part by the Nebraska Agricultural Research Division and Applied Carotenoid Sciences, Inc.).",
author = "J. Sun and Giraud, {D. W.} and Moxley, {Rodney A} and Driskell, {J. A.}",
year = "1996",
month = "12",
day = "1",
language = "English (US)",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - β-Carotene and α-tocopherol inhibit the development of atherosclerotic lesions in hypercholesterolemic rabbits

AU - Sun, J.

AU - Giraud, D. W.

AU - Moxley, Rodney A

AU - Driskell, J. A.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Male New Zealand rabbits were made hypercholerolemic by feeding an atherogenic diet (0.5% cholesterol, 3% peanut oil, and 3% coconut oil) with or without antioxidants for 8 weeks. The treatments were IV injection of β-carotene (25 mg/kg BW, twice weekly), dietary α-tocopherol (0.5%), a combination of both, and no added antioxidants (control), β-carotene treatment significantly decreased total and LDL cholesterol concentrations, thoracic atherosclerotic lesion area, and aortic intimai thickness, but had no effects on LDL oxidation ex vivo as compared to control. Dietary a-tocopherol supplementation significantly decreased the susceptibility of LDL to oxidation ex vivo, total atherosclerotic lesion area, and aortic intimai thickness as compared to control. Combination of both antioxidants significantly decreased the susceptibility of LDL to oxidation ex vivo, total atherosclerotic lesion area, and aortic intimai thickness as compared to control, but not β-carotene or α-tocopherol groups. These data suggest that the inhibition of development of atherosclerotic lesions by antioxidants may result from a combination of both antioxidant and nonantioxidant activities. (Supported in part by the Nebraska Agricultural Research Division and Applied Carotenoid Sciences, Inc.).

AB - Male New Zealand rabbits were made hypercholerolemic by feeding an atherogenic diet (0.5% cholesterol, 3% peanut oil, and 3% coconut oil) with or without antioxidants for 8 weeks. The treatments were IV injection of β-carotene (25 mg/kg BW, twice weekly), dietary α-tocopherol (0.5%), a combination of both, and no added antioxidants (control), β-carotene treatment significantly decreased total and LDL cholesterol concentrations, thoracic atherosclerotic lesion area, and aortic intimai thickness, but had no effects on LDL oxidation ex vivo as compared to control. Dietary a-tocopherol supplementation significantly decreased the susceptibility of LDL to oxidation ex vivo, total atherosclerotic lesion area, and aortic intimai thickness as compared to control. Combination of both antioxidants significantly decreased the susceptibility of LDL to oxidation ex vivo, total atherosclerotic lesion area, and aortic intimai thickness as compared to control, but not β-carotene or α-tocopherol groups. These data suggest that the inhibition of development of atherosclerotic lesions by antioxidants may result from a combination of both antioxidant and nonantioxidant activities. (Supported in part by the Nebraska Agricultural Research Division and Applied Carotenoid Sciences, Inc.).

UR - http://www.scopus.com/inward/record.url?scp=33749151564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749151564&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -