β-Adrenergic agonists attenuate fibroblast-mediated contraction of released collagen gels

Tadashi Mio, Yuichi Adachi, Stefano Carnevali, Debra Romberger, John R. Spurzem, Stephen I. Rennard

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The effects of β-adrenergic agonists on fibroblast-mediated collagen gel contraction were investigated. β-Agonists isoproterenol and epinephrine significantly attenuated fibroblast-mediated gel contraction in a concentration-dependent manner, whereas α-agonist norepinephrine had no effect. The biologically active form of isoproterenol, (-)-isoproterenol, was 10-fold more effective than the optical isoform, (+)-isoproterenol. β- Antagonists sotalol and propranolol reversed the attenuation caused by 10- 7 M isoproterenol or epinephrine at the concentration of 10-7 M or 10-6 M, but the α-antagonist phentolamine did not. However, β1- or β2- specificity of these effects is not clear. Isobutyl methylxanthine augmented the effect of isoproterenol and also prolonged the duration. Two reagents which are known to increase intracellular adenosine 3',5'-cyclic monophosphate (cAMP), prostaglandin E2 and dibutyryl adenosine 3',5'-cyclic monophosphate, attenuated gel contraction in a concentration-dependent manner. These data suggest that the fibroblast-mediated collagen gel contraction can be modulated by β-adrenergic agonists and that the effect depends on cAMP.

Original languageEnglish (US)
Pages (from-to)L829-L835
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume270
Issue number5 14-5
StatePublished - May 1 1996

Fingerprint

Adrenergic Agonists
Isoproterenol
Collagen
Fibroblasts
Gels
Epinephrine
Sotalol
Bucladesine
Phentolamine
Dinoprostone
Propranolol
Cyclic AMP
Adrenergic Agents
Norepinephrine
Protein Isoforms

Keywords

  • dibutyryl adenosine 3',5'-cyclic monophosphate
  • isoproterenol
  • salmeterol
  • terbutaline
  • wound retraction

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

β-Adrenergic agonists attenuate fibroblast-mediated contraction of released collagen gels. / Mio, Tadashi; Adachi, Yuichi; Carnevali, Stefano; Romberger, Debra; Spurzem, John R.; Rennard, Stephen I.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 270, No. 5 14-5, 01.05.1996, p. L829-L835.

Research output: Contribution to journalArticle

Mio, Tadashi ; Adachi, Yuichi ; Carnevali, Stefano ; Romberger, Debra ; Spurzem, John R. ; Rennard, Stephen I. / β-Adrenergic agonists attenuate fibroblast-mediated contraction of released collagen gels. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1996 ; Vol. 270, No. 5 14-5. pp. L829-L835.
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