α-Cell neogenesis in an animal model of IDDM

Lorraine A. O'Reilly, Danling Gu, Nora E Sarvetnick, Helena Edlund, Jenny M. Phillips, Tony Fulford, Anne Cooke

Research output: Contribution to journalArticle

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Abstract

Currently there is debate regarding the capacity of pancreatic islets to regenerate in adult animals. Because pancreatic endocrine cells are thought to arise from duct cells, we examined the pancreatic ductal epithelium of the diabetic NOD mouse for evidence of islet neogenesis. We have evidence of duct proliferation as well as ductal cell differentiation, as suggested by bromodeoxyuridine-labeling and the presence of glucagon-containing cells within these ducts. In addition, the ductal epithelia in diabetic NOD mice expressed the neuroendocrine markers neuropeptide Y and tyrosine hydroxylase. These ducts also expressed the homeobox gene product, insulin promoter factor 1. Ductal cell proliferation and expression of these markers was not observed in transgenic NOD mice (NOD-E), which do not develop clinical or histopathological symptoms of IDDM. This suggests that the observed ductal cell proliferation and differentiation was a direct result of β-cell destruction and insulin insufficiency in these adult diabetic mice, which further suggests that these events are recapitulating islet ontogeny observed during embryogenesis. It is possible that comparable processes occur in the human diabetic pancreas.

Original languageEnglish (US)
Pages (from-to)599-606
Number of pages8
JournalDiabetes
Volume46
Issue number4
StatePublished - Apr 1 1997

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Inbred NOD Mouse
Type 1 Diabetes Mellitus
Animal Models
Neuropeptide Y
Cell Differentiation
Epithelium
Cell Proliferation
Endocrine Cells
Homeobox Genes
Tyrosine 3-Monooxygenase
Bromodeoxyuridine
Glucagon
Islets of Langerhans
Transgenic Mice
Embryonic Development
Pancreas
Insulin

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

O'Reilly, L. A., Gu, D., Sarvetnick, N. E., Edlund, H., Phillips, J. M., Fulford, T., & Cooke, A. (1997). α-Cell neogenesis in an animal model of IDDM. Diabetes, 46(4), 599-606.

α-Cell neogenesis in an animal model of IDDM. / O'Reilly, Lorraine A.; Gu, Danling; Sarvetnick, Nora E; Edlund, Helena; Phillips, Jenny M.; Fulford, Tony; Cooke, Anne.

In: Diabetes, Vol. 46, No. 4, 01.04.1997, p. 599-606.

Research output: Contribution to journalArticle

O'Reilly, LA, Gu, D, Sarvetnick, NE, Edlund, H, Phillips, JM, Fulford, T & Cooke, A 1997, 'α-Cell neogenesis in an animal model of IDDM', Diabetes, vol. 46, no. 4, pp. 599-606.
O'Reilly LA, Gu D, Sarvetnick NE, Edlund H, Phillips JM, Fulford T et al. α-Cell neogenesis in an animal model of IDDM. Diabetes. 1997 Apr 1;46(4):599-606.
O'Reilly, Lorraine A. ; Gu, Danling ; Sarvetnick, Nora E ; Edlund, Helena ; Phillips, Jenny M. ; Fulford, Tony ; Cooke, Anne. / α-Cell neogenesis in an animal model of IDDM. In: Diabetes. 1997 ; Vol. 46, No. 4. pp. 599-606.
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