Heterodisulfide Reductase in Methanosarcina acetivorans

Project: Research project

Description

DESCRIPTION (provided by applicant): Methane production is critically important to a number of human-related problems including global warming, waste treatment, and the development of alternative fuels from biological materials. The bulk of methane on earth is produced by methanogenic archaea through the mechanism known as methanogenesis. An essential step in methanogenesis is regeneration of a CoB-CoM heterodi- sulfide formed upon methane production. The enzyme complex responsible for CoB-CoM reduction is known as heterodisulfide reductcase (Hdr). The genome of Methanosarcina acetivorans contains ORFs coding for a soluble HdrABC complex and a membrane-bound HdrDE complex. Whether HdrABC and HdrDE have specialized, or completely redundant functions, will be tested by creating reporter gene fusions, targeted gene deletions, and chimeric Hdr complexes. In vitro analysis of chimeric enzyme complexes will be used to identify amino acid residues important for specific and selective protein:protein interactions. Through these experiments, the mechanism of methanogenesis and the archaeal electron transport system will be elucidated, thereby gaining insight into the processes of energy generation and conservation that are essential to all forms of life.
StatusFinished
Effective start/end date11/16/0611/15/09

Funding

  • National Institutes of Health: $43,996.00
  • National Institutes of Health: $46,826.00
  • National Institutes of Health: $50,054.00

Fingerprint

methane production
Methanosarcina
Methane
alternative fuels
waste treatment
methanogens
gene fusion
gene deletion
protein-protein interactions
Archaea
enzymes
sulfides
electron transfer
methane
Global Warming
Gene Fusion
Gene Deletion
Sulfides
Enzymes
Electron Transport

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)