William H Velander, PhD, MChE

Professor

  • 1666 Citations
  • 24 h-Index
1987 …2019
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Research Output 1987 2019

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Article
2019

Co-expressing Turnip Crinkle Virus-coat protein with the serine protease α-thrombin precursor (pFIIa) in Nicotiana benthamiana Domin

Laguia-Becher, M., Zaldúa, Z., Xu, W., Marconi, P. L., Velander, W. & Alvarez, M. A., Feb 15 2019, In : In Vitro Cellular and Developmental Biology - Plant. 55, 1, p. 88-98 11 p.

Research output: Contribution to journalArticle

Carmovirus
Turnip crinkle virus
thrombin
Nicotiana benthamiana
Capsid Proteins
2018
1 Citation (Scopus)

A totally recombinant fibrin matrix for mesenchymal stem cell culture and delivery

Wang, O., Ismail, A., Fabian, F. M., Lin, H., Li, Q., Elowsky, C., Carlson, M. A., Burgess, W., Velander, W. H., Kidambi, S. S. & Lei, Y., Dec 2018, In : Journal of Biomedical Materials Research - Part A. 106, 12, p. 3135-3142 8 p.

Research output: Contribution to journalArticle

Stem cells
Fibrin
Cell culture
Fibrinogen
Clinical laboratories

Fluid administration rate for uncontrolled intraabdominal hemorrhage in swine

Yanala, U. R., Johanning, J. M., Pipinos, I. I., High, R. R., Larsen, G., Velander, W. H. & Carlson, M. A., Nov 2018, In : PloS one. 13, 11, e0207708.

Research output: Contribution to journalArticle

hemorrhage
Resuscitation
hemoglobin
Hemoglobins
Blood

The isolation of a plasma-derived γγ’ fibrinogen: Fibronectin mixture that forms a novel polymeric matrix

Ismail, A. E. A., Fabian, F. M., Wang, O., Lei, Y., Carlson, M. A., Burgess, W. H. & Velander, W. H., Dec 2018, In : Process Biochemistry. 75, p. 257-265 9 p.

Research output: Contribution to journalArticle

Fibronectins
Fibrinogen
Plasmas
Fibrin
Plasma (human)
2015
6 Citations (Scopus)

Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk

Zhao, J., Xu, W., Ross, J. W., Walters, E. M., Butler, S. P., Whyte, J. J., Kelso, L., Fatemi, M., Vanderslice, N. C., Giroux, K., Spate, L. D., Samuel, M. S., Murphy, C. N., Wells, K. D., Masiello, N. C., Prather, R. S. & Velander, W. H., Sep 21 2015, In : Scientific reports. 5, 14176.

Research output: Contribution to journalArticle

Hemophilia B
Protein Engineering
Factor IX
Human Mammary Glands
Milk
2 Citations (Scopus)

Quantifying vitamin K-dependent holoprotein compaction caused by differential γ-carboxylation using high-pressure size exclusion chromatography

Vanderslice, N. C., Messer, A. S., Vadivel, K., Bajaj, S. P., Phillips, M., Fatemi, M., Xu, W. & Velander, W. H., Jun 15 2015, In : Analytical Biochemistry. 479, p. 6-14 9 p.

Research output: Contribution to journalArticle

Carboxylation
Size exclusion chromatography
Vitamin K
Gel Chromatography
Compaction
2014
15 Citations (Scopus)

A totally recombinant human fibrin sealant

Carlson, M. A., Calcaterra, J., Johanning, J. M., Pipinos, I. I., Cordes, C. M. & Velander, W. H., Mar 1 2014, In : Journal of Surgical Research. 187, 1, p. 334-342 9 p.

Research output: Contribution to journalArticle

Fibrin Tissue Adhesive
Thrombelastography
Hemostatics
Fibrinogen
Swine
2 Citations (Scopus)
Liver
hemorrhage
Swine
Hemorrhage
liver
2013
16 Citations (Scopus)

Recombinant human fibrinogen that produces thick fibrin fibers with increased wound adhesion and clot density

Calcaterra, J., Van Cott, K., Butler, S. P., Gil, G. C., Germano, M., Van Veen, H. A., Nelson, K., Forsberg, E. J., Carlson, M. A. & Velander, W. H., Jan 14 2013, In : Biomacromolecules. 14, 1, p. 169-178 10 p.

Research output: Contribution to journalArticle

Fibrin
Fibrinogen
Adhesion
Plasmas
Fibers
2011
8 Citations (Scopus)

Functional factor VIII made with von Willebrand factor at high levels in transgenic milk

Pipe, S. W., Miao, H., Butler, S. P., Calcaterra, J. & Velander, W. H., Nov 1 2011, In : Journal of Thrombosis and Haemostasis. 9, 11, p. 2235-2242 8 p.

Research output: Contribution to journalArticle

Factor VIII
von Willebrand Factor
Milk
Hemophilia A
Furin
2010
39 Citations (Scopus)
N-Acetylneuraminic Acid
Ionization
Mass spectrometry
Polysaccharides
Desorption
2009
25 Citations (Scopus)

N-glycosylation microheterogeneity and site occupancy of an Asn-X-Cys sequon in plasma-derived and recombinant protein C

Gil, G. C., Velander, W. H. & Van Cott, K. E., May 1 2009, In : Proteomics. 9, 9, p. 2555-2567 13 p.

Research output: Contribution to journalArticle

Glycosylation
Protein C
Recombinant Proteins
Polysaccharides
Blood Proteins
2008
26 Citations (Scopus)

Analysis of the N-glycans of recombinant human Factor IX purified from transgenic pig milk

Gil, G. C., Velander, W. H. & Van Cott, K. E., Jul 1 2008, In : Glycobiology. 18, 7, p. 526-539 14 p.

Research output: Contribution to journalArticle

Factor IX
Human engineering
Polysaccharides
Milk
Swine
22 Citations (Scopus)

Construction and characterization of a thrombin-resistant designer FGF-based collagen binding domain angiogen

Brewster, L. P., Washington, C., Brey, E. M., Gassman, A., Subramanian, A., Calceterra, J., Wolf, W., Hall, C. L., Velander, W. H., Burgess, W. H. & Greisler, H. P., Jan 1 2008, In : Biomaterials. 29, 3, p. 327-336 10 p.

Research output: Contribution to journalArticle

Fibroblast Growth Factor 1
Fibroblast Growth Factors
Fibroblasts
Collagen
Thrombin
2006
1 Citation (Scopus)

Purificación del factor IX recombinante expresado en leche de cerdas transgénicas

Translated title of the contribution: Purification of recombinant factor IX expressed in milk of transgenic sowsLahens, A. C., Torres, A. M., Velander, W., Vidal, M. L., Sánchez, L. J., Rojas, A. R., Tito, L. S., Concepción, A. D., Almagro, P. C., González, D. C. & Vázquez, D. A., May 1 2006, In : Revista Cubana de Hematologia, Inmunologia y Hemoterapia. 22, 2

Research output: Contribution to journalArticle

Factor IX
Milk
Hemophilia B
Ion Exchange
Ultracentrifugation
2004
25 Citations (Scopus)

Haemophilic factors produced by transgenic livestock: Abundance that can enable alternative therapies worldwide

Van Cott, K. E., Monahan, P. E., Nichols, T. C. & Velander, W. H., Oct 1 2004, In : Haemophilia, Supplement. 10, 4, p. 70-76 7 p.

Research output: Contribution to journalArticle

Livestock
Complementary Therapies
Hemophilia A
Milk
Swine
34 Citations (Scopus)

Purification of recombinant DNA-derived factor IX produced in transgenic pig milk and fractionation of active and inactive subpopulations

Lindsay, M., Gil, G. C., Cadiz, A., Velander, W. H., Zhang, C. & Van Cott, K. E., Feb 13 2004, In : Journal of Chromatography A. 1026, 1-2, p. 149-157 9 p.

Research output: Contribution to journalArticle

Factor IX
Recombinant DNA
Fractionation
Purification
Milk
11 Citations (Scopus)

Secretion of recombinant human fibrinogen by the murine mammary gland

Butler, S., O'Sickey, T., Lord, S., Lubon, H., Gwazdauskas, F. & Velander, W., Oct 1 2004, In : Transgenic Research. 13, 5, p. 437-450 14 p.

Research output: Contribution to journalArticle

fibrinogen
Human Mammary Glands
mammary glands
Fibrinogen
secretion
2003

Development of murine embryos following electroporation

Schmotzer, C. A., Dunlap-Brown, M. E., Butler, S. P., Velander, W. H. & Gwazdauskas, F. C., Apr 1 2003, In : Journal of Assisted Reproduction and Genetics. 20, 4, p. 148-152 5 p.

Research output: Contribution to journalArticle

Electroporation
Zygote
Embryonic Development
Embryonic Structures
Electric Stimulation
2002
22 Citations (Scopus)

Scale-up of microbubble dispersion generator for aerobic fermentation

Hensirisak, P., Parasukulsatid, P., Agblevor, F. A., Cundiff, J. S. & Velander, W. H., Jul 2 2002, In : Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology. 101, 3, p. 211-227 17 p.

Research output: Contribution to journalArticle

Microbubbles
Fermentation
Air
Microorganisms
Yeast
2001
30 Citations (Scopus)

Recombinant human protein C expression in the milk of transgenic pigs and the effect on endogenous milk immunoglobulin and transferrin levels

Cott, K. E. V., Lubon, H., Gwazdauskas, F. C., Knight, J., Drohan, W. N. & Velander, W. H., Mar 12 2001, In : Transgenic Research. 10, 1, p. 43-51 9 p.

Research output: Contribution to journalArticle

transferrin
Transferrin
Protein C
Recombinant Proteins
immunoglobulins
2000
11 Citations (Scopus)

Interferometric optical fiber microcantilever beam biosensor

Wavering, T., Meller, S., Evans, M., Pennington, C., Jones, M., Van Tassell, R., Murphy, K., Velander, W. & Valdes, E., Jan 1 2000, In : Proceedings of SPIE - The International Society for Optical Engineering. 4200, p. 10-16 7 p.

Research output: Contribution to journalArticle

Biosensor
bioinstrumentation
Biosensors
Optical Fiber
Optical fibers
3 Citations (Scopus)

Ranking the factors impacting immunosorbent performance

Subramanian, A. & Velander, W. H., Jan 1 2000, In : International Journal of Bio-Chromatography. 5, 1, p. 67-82 16 p.

Research output: Contribution to journalArticle

Immunosorbents
Immobilization
Immobilized Antibodies
Antigens
Antibodies
1999
22 Citations (Scopus)

Bovine Follicular Dynamics, Oocyte Recovery, and Development of Oocytes Microinjected with a Green Fluorescent Protein Construct

Chauhan, M. S., Nadir, S., Bailey, T. L., Pryor, A. W., Butler, S. P., Notter, D. R., Velander, W. H. & Gwazdauskas, F. C., May 1999, In : Journal of Dairy Science. 82, 5, p. 918-926 9 p.

Research output: Contribution to journalArticle

Open Access
pronucleus
Green Fluorescent Proteins
green fluorescent protein
Oocytes
oocytes
2 Citations (Scopus)

In vitro embryo development of good and poor quality bovine oocytes following microinjection of DNA

Garst, A. S., Carlin, S. K., Glbbons, J. R., Kendrick, K. W., Pearson, R. E., Akers, R. M., Bailey, T. L., Velander, W. H., Lubon, H. K. & Gwazdauskas, F. C., Jan 1 1999, In : Transgenics. 2, 4, p. 371-382 12 p.

Research output: Contribution to journalArticle

Microinjections
Oocytes
Embryonic Development
Abattoirs
DNA
36 Citations (Scopus)

Transgenic pigs as bioreactors: A comparison of gamma-carboxylation of glutamic acid in recombinant human protein C and factor IX by the mammary gland

Van Cott, K. E., Butler, S. P., Russell, C. G., Subramanian, A., Lubon, H., Gwazdauskas, F. C., Knight, J., Drohan, W. N. & Velander, W. H., Nov 1 1999, In : Genetic Analysis - Biomolecular Engineering. 15, 3-5, p. 155-160 6 p.

Research output: Contribution to journalArticle

Carboxylation
Coagulants
Factor IX
Bioreactors
Human Mammary Glands
1998
18 Citations (Scopus)

Zn2+-selective purification of recombinant proteins from the milk of transgenic animals

Degener, A., Belew, M. & Velander, W. H., Mar 13 1998, In : Journal of Chromatography A. 799, 1-2, p. 125-137 13 p.

Research output: Contribution to journalArticle

Genetically Modified Animals
Recombinant Proteins
Purification
Milk
Animals
1997
20 Citations (Scopus)

Current progress in the production of recombinant human fibrinogen in the milk of transgenic animals

Butler, S. P., Van Cott, K., Subrumanian, A., Gwazduaskas, F. C. & Velander, W. H., Jul 1 1997, In : Thrombosis and Haemostasis. 78, 1, p. 537-542 6 p.

Research output: Contribution to journalArticle

Genetically Modified Animals
Fibrinogen
Milk
Human Mammary Glands
Breast
29 Citations (Scopus)

Dot-blot analysis of the degree of covalent modification of proteins and antibodies at amino groups

Morçöl, T., Subramanian, A. & Velander, W. H., Apr 11 1997, In : Journal of Immunological Methods. 203, 1, p. 45-53 9 p.

Research output: Contribution to journalArticle

Trinitrobenzenesulfonic Acid
Antibodies
Proteins
Membranes
Sample Size
24 Citations (Scopus)

Phenotypic and genotypic stability of multiple lines of transgenic pigs expressing recombinant human protein C

Van Cott, K. E., Lubon, H., Russell, C. G., Butler, S. P., Gwazdauskas, F. C., Knight, J., Drohan, W. N. & Velander, W. H., Jan 1 1997, In : Transgenic Research. 6, 3, p. 203-212 10 p.

Research output: Contribution to journalArticle

genetic stability
Protein C
Recombinant Proteins
Swine
transgenes
28 Citations (Scopus)

Transgenic livestock as drug factories

Velander, W. H., Lubon, H. & Drohan, W. N., Jan 1 1997, In : Scientific American. 276, 1, p. [d]70-74

Research output: Contribution to journalArticle

Livestock
Genetic Engineering
Pharmaceutical Preparations
Sheep
Milk
101 Citations (Scopus)

Transgenic pigs produce functional human factor VIII in milk

Paleyanda, R. K., Velander, W. H., Lee, T. K., Scandella, D. H., Gwazdauskas, F. C., Knight, J. W., Hoyer, L. W., Drohan, W. N. & Lubon, H., Oct 1997, In : Nature Biotechnology. 15, 10, p. 971-975 5 p.

Research output: Contribution to journalArticle

Plasma (human)
Factor VIII
Human engineering
Assays
Milk
1996
9 Citations (Scopus)

Activation of recombinant human protein C

Lee, T. K., Bangalore, N., Velander, W., Drohan, W. N. & Lubon, H., May 1 1996, In : Thrombosis Research. 82, 3, p. 225-234 10 p.

Research output: Contribution to journalArticle

Protein C
Recombinant Proteins
Thrombin
Thrombomodulin
Enzyme Precursors
20 Citations (Scopus)

Affinity Purification of Biologically Active and Inactive Forms of Recombinant Human Protein C Produced in Porcine Mammary Gland

Van Cott, K. E., Williams, B., Velander, W. H., Gwazdauskas, F., Lee, T., Lubon, H. & Drohan, W. N., Jan 1 1996, In : Journal of Molecular Recognition. 9, 5-6, p. 407-414 8 p.

Research output: Contribution to journalArticle

Human Mammary Glands
Protein C
Recombinant Proteins
Purification
Swine
23 Citations (Scopus)

Effect of Antibody Orientation on Immunosorbent Performance

Subramanian, A. & Velander, W. H., Jan 1 1996, In : Journal of Molecular Recognition. 9, 5-6, p. 528-535 8 p.

Research output: Contribution to journalArticle

Immunosorbents
Monoclonal antibodies
Antibodies
Monoclonal Antibodies
Immobilization
1 Citation (Scopus)

Human Plasma Proteins from Transgenic Animal Bioreactors

Paleyanda, R. K., Velander, W. H., Lee, T. K., Drews, R., Gwazdauskas, F. C., Knight, J. W., Drohan, W. N. & Lubon, H., Dec 1 1996, In : ACS Symposium Series. 647, p. 205-217 13 p.

Research output: Contribution to journalArticle

Plasma (human)
Bioreactors
Blood Proteins
Animals
Protein C
36 Citations (Scopus)

Proteins from transgenic animal bioreactors

Lubon, H., Paleyanda, R. K., Velander, W. H. & Drohan, W. N., Jan 1 1996, In : Transfusion Medicine Reviews. 10, 2, p. 131-143 13 p.

Research output: Contribution to journalArticle

Recombinant Fusion Proteins
Species Specificity
Animal Mammary Glands
Genetically Modified Animals
Protein Precursors
11 Citations (Scopus)

Rate limitations in posttranslational processing by the mammary gland of transgenic animals

Subramanian, A., Paleyanda, R. K., Lubon, H., Williams, B. L., Gwazdauskas, F. C., Knight, J. W., Drohan, W. N. & Velander, W. H., Jan 1 1996, In : Annals of the New York Academy of Sciences. 782, p. 87-96 10 p.

Research output: Contribution to journalArticle

Genetically Modified Animals
Human Mammary Glands
Protein C
Animals
Recombinant Proteins
1995
15 Citations (Scopus)

Transgene detection during early murine embryonic development after pronuclear microinjection

Page, R. L., Canseco, R. S., Russell, C. G., Johnson, J. L., Velander, W. H. & Gwazdauskas, F. C., Jan 1 1995, In : Transgenic Research. 4, 1, p. 12-17 6 p.

Research output: Contribution to journalArticle

Microinjections
Transgenes
transgenes
Embryonic Development
embryogenesis
34 Citations (Scopus)

Transgenesis in mice by cytoplasmic injection of polylysine/DNA mixtures

Page, R. L., Butler, S. P., Subramanian, A., Gwazdauskas, F. C., Johnson, J. L. & Velander, W. H., Nov 1 1995, In : Transgenic Research. 4, 6, p. 353-360 8 p.

Research output: Contribution to journalArticle

Gene Transfer Techniques
Polylysine
injection
Injections
DNA
1994
2 Citations (Scopus)

Development of bovine morulae after bisection and biopsy and assessment of DNA amplification by the polymerase chain reaction

Sparks, A. E. T., Canseco, R. S., Russell, C. G., Johnson, J. L., Page, J. L., Velander, W. H., McGilliard, M. L. & Gwazdauskas, F. C., Mar 1994, In : Animal Reproduction Science. 35, 1-2, p. 1-7 7 p.

Research output: Contribution to journalArticle

Morula
morula
DNA-Directed DNA Polymerase
biopsy
embryo (animal)
16 Citations (Scopus)

Effect of culture conditions, donor age, and injection site on in vitro development of DNA microinjected porcine zygotes.

Hajdu, M. A., Knight, J. W., Canseco, R. S., Krisher, R. L., Velander, W. H., Pearson, R. E. & Gwazdauskas, F. C., May 1994, In : Journal of animal science. 72, 5, p. 1299-1305 7 p.

Research output: Contribution to journalArticle

Zygote
injection site
zygote
Swine
Injections
4 Citations (Scopus)

Effects of Time of Deoxyribonucleic Acid Microinjection on Gene Detection and In Vitro Development of Bovine Embryos

Sparks, A. E. T., Canseco, R. S., Russell, C. G., Johnson, J. L., Moll, H. D., Velander, W. H. & Gwazdauskas, F. C., Jan 1 1994, In : Journal of Dairy Science. 77, 3, p. 718-724 7 p.

Research output: Contribution to journalArticle

Open Access
Microinjections
Embryonic Development
embryo (animal)
Embryonic Structures
cattle
287 Citations (Scopus)

Expression of a functional human complement inhibitor in a transgenic pig as a model for the prevention of xenogeneic hyperacute organ rejection

Fodor, W. L., Williams, B. L., Matis, L. A., Madri, J. A., Rollins, S. A., Knight, J. W., Velander, W. & Squnito, S. P., Nov 8 1994, In : Proceedings of the National Academy of Sciences of the United States of America. 91, 23, p. 11153-11157 5 p.

Research output: Contribution to journalArticle

Complement Inactivating Agents
Swine
Organ Transplantation
Heterologous Transplantation
Vascular Endothelium
31 Citations (Scopus)

Gene transfer efficiency during gestation and the influence of co-transfer of non-manipulated embryos on production of transgenic mice

Canseco, R. S., Sparks, A. E. T., Page, R. L., Russell, C. G., Johnson, J. L., Velander, W. H., Pearson, R. E., Drohan, W. N. & Gwazdauskas, F. C., Jan 1 1994, In : Transgenic Research. 3, 1, p. 20-25 6 p.

Research output: Contribution to journalArticle

gene transfer
Transgenic Mice
embryo (animal)
Embryonic Structures
pregnancy
38 Citations (Scopus)

Inefficient processing of human protein C in the mouse mammary gland

Drohan, W. N., Zhang, D. W., Paleyanda, R., Chang, R., Wroble, M., Velander, W. & Lubon, H., Nov 1 1994, In : Transgenic Research. 3, 6, p. 355-364 10 p.

Research output: Contribution to journalArticle

Human Mammary Glands
Protein C
mammary glands
Recombinant Proteins
mice
18 Citations (Scopus)

Influence of time of gene microinjection on development and DNA detection frequency in bovine embryos

Krisher, R. L., Gibbons, J. R., Canseco, R. S., Johnson, J. L., Russell, C. G., Notter, D. R., Velander, W. H. & Gwazdauskas, F. C., Jul 1 1994, In : Transgenic Research. 3, 4, p. 226-231 6 p.

Research output: Contribution to journalArticle

Microinjections
Insemination
embryo (animal)
Embryonic Structures
insemination
18 Citations (Scopus)

Ovulation rate, zygote recovery and follicular populations in FSH-superovulated goats treated with PGF and/or GnRH

Krisher, R. L., Gwazdauskas, F. C., Page, R. L., Russell, C. G., Canseco, R. S., Sparks, A. E. T., Velander, W. H., Johnson, J. L. & Pearson, R. E., Feb 1994, In : Theriogenology. 41, 2, p. 491-498 8 p.

Research output: Contribution to journalArticle

Dinoprost
Zygote
follicle-stimulating hormone
Follicle Stimulating Hormone
zygote
26 Citations (Scopus)

Role of local antibody density effects on immunosorbent efficiency

Subramanian, A., Van Cott, K. E., Milbrath, D. S. & Velander, W. H., Jun 24 1994, In : Journal of Chromatography A. 672, 1-2, p. 11-24 14 p.

Research output: Contribution to journalArticle

Immunosorbents
Antibodies
Immobilized Antibodies
Permeation
Antigens
26 Citations (Scopus)

The Porcine Mammary Gland as a Bioreactor for Complex Proteinsa

MORCÖL, TÜLIN., AKERS, ROBERT. M., JOHNSON, JOHN. L., WILLIAMS, BARRY. L., GWAZDAUSKAS, FRANCIS. C., KNIGHT, JAMES. W., LUBON, HENRYK., PALEYANDA, REKHA. K., DROHAN, WILLIAM. N. & VELANDER, WILLIAM. H., Jun 1994, In : Annals of the New York Academy of Sciences. 721, 1, p. 218-233 16 p.

Research output: Contribution to journalArticle

Bioreactors
Human Mammary Glands
Swine
Bioactivity
Cell culture