Lawrence M Schopfer, PhD

Research Assistant Professor

  • 4221 Citations
  • 37 h-Index
1975 …2019
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Research Output 1975 2019

  • 4221 Citations
  • 37 h-Index
  • 137 Article
  • 3 Chapter
  • 3 Review article
  • 1 Comment/debate
2019

Computer-designed active human butyrylcholinesterase double mutant with a new catalytic triad

Grigorenko, B. L., Novichkova, D. A., Lushchekina, S. V., Zueva, I. V., Schopfer, L. M., Nemukhin, A. V., Varfolomeev, S. D., Lockridge, O. & Masson, P., Jun 1 2019, In : Chemico-Biological Interactions. 306, p. 138-146 9 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Organophosphates
Enzymes
Electrophoresis
Cell culture

Half-life of chlorpyrifos oxon and other organophosphorus esters in aqueous solution

Lockridge, O., Verdier, L. & Schopfer, L. M., Sep 25 2019, In : Chemico-Biological Interactions. 311, 108788.

Research output: Contribution to journalArticle

Open Access
Half-Life
Esters
Crosslinking
O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate
Artifacts
2 Citations (Scopus)
Lysine
Mass spectrometry
Mass Spectrometry
Tubulin
Proteins
4 Citations (Scopus)
Butyrylcholinesterase
Affinity chromatography
cholinesterase
Ion Exchange
ion exchange
2018
2 Citations (Scopus)

Characterization of butyrylcholinesterase from porcine milk

Saxena, A., Belinskaya, T., Schopfer, L. M. & Lockridge, O., Aug 15 2018, In : Archives of Biochemistry and Biophysics. 652, p. 38-49 12 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Milk
Swine
Butyrylthiocholine
Acetylthiocholine
2 Citations (Scopus)
Tubulin
Agglomeration
Lysine
Molecular Weight
Molecular weight
3 Citations (Scopus)

Cryo-EM structure of the native butyrylcholinesterase tetramer reveals a dimer of dimers stabilized by a superhelical assembly

Leung, M. R., van Bezouwen, L. S., Schopfer, L. M., Sussman, J. L., Silman, I., Lockridge, O. & Zeev-Ben-Mordehai, T., Dec 26 2018, In : Proceedings of the National Academy of Sciences of the United States of America. 115, 52, p. 13270-13275 6 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Cryoelectron Microscopy
Cholinesterases
Proline
Catalytic Domain
4 Citations (Scopus)

Mass Spectral Detection of Diethoxyphospho-Tyrosine Adducts on Proteins from HEK293 Cells Using Monoclonal Antibody depY for Enrichment

Onder, S., Schopfer, L. M., Tacal, O., Blake, T. A., Johnson, R. C. & Lockridge, O., Jun 18 2018, In : Chemical Research in Toxicology. 31, 6, p. 520-530 11 p.

Research output: Contribution to journalArticle

HEK293 Cells
Tyrosine
Monoclonal Antibodies
Peptides
Proteins
7 Citations (Scopus)

Optimization of cholinesterase-based catalytic bioscavengers against organophosphorus agents

Lushchekina, S. V., Schopfer, L. M., Grigorenko, B. L., Nemukhin, A. V., Varfolomeev, S. D., Lockridge, O. & Masson, P., Mar 13 2018, In : Frontiers in Pharmacology. 9, MAR, 211.

Research output: Contribution to journalArticle

Cholinesterases
Water
Enzymes
Hydrolases
Mutation
1 Citation (Scopus)

Purification of recombinant human butyrylcholinesterase on Hupresin®

Lockridge, O., David, E., Schopfer, L. M., Masson, P., Brazzolotto, X. & Nachon, F., Dec 1 2018, In : Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 1102-1103, p. 109-115 7 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Purification
Procainamide
Acetylcholinesterase
Sepharose
cholinesterase
Sus scrofa
mass spectrometry
peptides
milk
2 Citations (Scopus)

Use of Hupresin to Capture Red Blood Cell Acetylcholinesterase for Detection of Soman Exposure

Onder, S., Schopfer, L. M., Cashman, J. R., Tacal, O., Johnson, R. C., Blake, T. A. & Lockridge, O., Jan 2 2018, In : Analytical chemistry. 90, 1, p. 974-979 6 p.

Research output: Contribution to journalArticle

Soman
Acetylcholinesterase
Blood
Cells
Neurology
2017
4 Citations (Scopus)

Characterization of butyrylcholinesterase in bovine serum

Dafferner, A. J., Lushchekina, S., Masson, P., Xiao, G., Schopfer, L. M. & Lockridge, O., Mar 25 2017, In : Chemico-Biological Interactions. 266, p. 17-27 11 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Acetylcholinesterase
Serum
Peptides
Proteins
7 Citations (Scopus)

Hupresin retains binding capacity for butyrylcholinesterase and acetylcholinesterase after sanitation with sodium hydroxide

Onder, S., David, E., Tacal, O., Schopfer, L. M. & Lockridge, O., Oct 10 2017, In : Frontiers in Pharmacology. 8, OCT, 713.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Sodium Hydroxide
Sanitation
Acetylcholinesterase
Erythrocytes
4 Citations (Scopus)

Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure

Dafferner, A. J., Schopfer, L. M., Xiao, G., Cashman, J. R., Yerramalla, U., Johnson, R. C., Blake, T. A. & Lockridge, O., Oct 16 2017, In : Chemical Research in Toxicology. 30, 10, p. 1897-1910 14 p.

Research output: Contribution to journalArticle

Acetylcholinesterase
Blood
Erythrocytes
Cells
Soman
2 Citations (Scopus)

Monoclonal Antibody That Recognizes Diethoxyphosphotyrosine-Modified Proteins and Peptides Independent of Surrounding Amino Acids

Onder, S., Dafferner, A. J., Schopfer, L. M., Xiao, G., Yerramalla, U., Tacal, O., Blake, T. A., Johnson, R. C. & Lockridge, O., Dec 18 2017, In : Chemical Research in Toxicology. 30, 12, p. 2218-2228 11 p.

Research output: Contribution to journalArticle

Monoclonal Antibodies
Tyrosine
Amino Acids
Peptides
Dichlorvos
8 Citations (Scopus)

The C5 variant of the butyrylcholinesterase tetramer includes a noncovalently bound 60 kDa lamellipodin fragment

Schopfer, L. M., Delacour, H., Masson, P., Leroy, J., Krejci, E. & Lockridge, O., Jul 2017, In : Molecules. 22, 7, 1083.

Research output: Contribution to journalArticle

Butyrylcholinesterase
genes
fragments
muscle relaxants
proteins
2016
11 Citations (Scopus)

Origin of polyproline-rich peptides in human butyrylcholinesterase tetramers

Peng, H., Schopfer, L. M. & Lockridge, O., Nov 25 2016, In : Chemico-Biological Interactions. 259, p. 63-69 7 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Peptides
Proteins
Plasma (human)
Plasmas
7 Citations (Scopus)
Butyrylcholinesterase
CHO Cells
Plasmas
Peptides
Genes
2015

Biomarkers of Exposure to Organophosphorus Poisons: A New Motif for Covalent Binding to Tyrosine in Proteins That Have No Active Site Serine. A New Motif for Covalent Binding to Tyrosine in Proteins That Have No Active Site Serine

Lockridge, O., Schopfer, L. M. & Masson, P., Jan 21 2015, Handbook of Toxicology of Chemical Warfare Agents: Second Edition. Elsevier Inc., p. 953-965 13 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Poisons
Serine
Tyrosine
Catalytic Domain
Biomarkers
26 Citations (Scopus)

Pure human butyrylcholinesterase hydrolyzes octanoyl ghrelin to desacyl ghrelin

Schopfer, L. M., Lockridge, O. & Brimijoin, S., Dec 1 2015, In : General and Comparative Endocrinology. 224, p. 61-68 8 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
ghrelin
Ghrelin
cholinesterase
Hydrolysis
2014
22 Citations (Scopus)

Characterization of a novel BCHE "silent" allele: Point mutation (p.Val204Asp) causes loss of activity and prolonged apnea with suxamethonium

Delacour, H., Lushchekina, S., Mabboux, I., Bousquet, A., Ceppa, F., Schopfer, L. M., Lockridge, O. & Masson, P., Jul 23 2014, In : PloS one. 9, 7, e101552.

Research output: Contribution to journalArticle

suxamethonium
apnea
Butyrylthiocholine
Succinylcholine
Apnea
14 Citations (Scopus)

Characterization of a novel butyrylcholinesterase point mutation (p.Ala34Val), "silent" with mivacurium

Delacour, H., Lushchekina, S., Mabboux, I., Ceppa, F., Masson, P., Schopfer, L. M. & Lockridge, O., Dec 1 2014, In : Biochemical Pharmacology. 92, 3, p. 476-483 8 p.

Research output: Contribution to journalArticle

Butyrylthiocholine
Butyrylcholinesterase
Point Mutation
Substrates
Molecular Dynamics Simulation
15 Citations (Scopus)

Detection of cresyl phosphate-modified butyrylcholinesterase in human plasma for chemical exposure associated with aerotoxic syndrome

Schopfer, L. M., Masson, P., Lamourette, P., Simon, S. & Lockridge, O., Sep 15 2014, In : Analytical Biochemistry. 461, p. 17-26 10 p.

Research output: Contribution to journalArticle

Plasma (human)
Butyrylcholinesterase
Phosphates
Plasmas
Mass spectrometry
3 Citations (Scopus)
Phosphates
Oxygen
Butyrylcholinesterase
Jet engines
Plasmas
2013
1 Citation (Scopus)
Butyrylcholinesterase
Histidine
Phosphates
Catalytic Domain
Tritolyl Phosphates
26 Citations (Scopus)
Butyrylcholinesterase
Osmotic Pressure
Molecular Dynamics Simulation
Viscosity
Molecular dynamics
23 Citations (Scopus)

Inhibition pathways of the potent organophosphate CBDP with cholinesterases revealed by X-ray crystallographic snapshots and mass spectrometry

Carletti, E., Colletier, J. P., Schopfer, L. M., Santoni, G., Masson, P., Lockridge, O., Nachon, F. & Weik, M., Feb 18 2013, In : Chemical Research in Toxicology. 26, 2, p. 280-289 10 p.

Research output: Contribution to journalArticle

Organophosphates
Cholinesterases
Acetylcholinesterase
Mass spectrometry
Mass Spectrometry
12 Citations (Scopus)

Mass Spectrometry Method to Identify Aging Pathways of Sp- and Rp-Tabun Adducts on Human Butyrylcholinesterase Based on the Acid Labile P-N Bond

Jiang, W., Cashman, J. R., Nachon, F., Masson, P., Schopfer, L. M. & Lockridge, O., Apr 1 2013, In : Toxicological Sciences. 132, 2, p. 390-398 9 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Mass spectrometry
Dealkylation
Mass Spectrometry
Deamination
16 Citations (Scopus)

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of titanium oxide-enriched peptides for detection of aged organophosphorus adducts on human butyrylcholinesterase

Jiang, W., Murashko, E. A., Dubrovskii, Y. A., Podolskaya, E. P., Babakov, V. N., Mikler, J., Nachon, F., Masson, P., Schopfer, L. M. & Lockridge, O., Jan 1 2013, In : Analytical Biochemistry. 439, 2, p. 132-141 10 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
5 Citations (Scopus)

Newly observed spontaneous activation of ethephon as a butyrylcholinesterase inhibitor

Liyasova, M. S., Schopfer, L. M., Kodani, S., Lantz, S. R., Casida, J. E. & Lockridge, O., Mar 18 2013, In : Chemical Research in Toxicology. 26, 3, p. 422-431 10 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Chemical activation
Catalytic Domain
Serine
ethephon
10 Citations (Scopus)

PHOS-select iron affinity beads enrich peptides for the detection of organophosphorus adducts on albumin

Jiang, W., Dubrovskii, Y. A., Podolskaya, E. P., Murashko, E. A., Babakov, V., Nachon, F., Masson, P., Schopfer, L. M. & Lockridge, O., Dec 16 2013, In : Chemical Research in Toxicology. 26, 12, p. 1917-1925 9 p.

Research output: Contribution to journalArticle

Albumins
Iron
Peptides
Pepsin A
Plasma (human)
7 Citations (Scopus)

Polyclonal antibody to soman-tyrosine

Li, B., Duysen, E. G., Froment, M. T., Masson, P., Nachon, F., Jiang, W., Schopfer, L. M., Thiele, G. M., Klassen, L. W., Cashman, J., Williams, G. R. & Lockridge, O., Apr 15 2013, In : Chemical Research in Toxicology. 26, 4, p. 584-592 9 p.

Research output: Contribution to journalArticle

Soman
Tyrosine
Antibodies
Albumins
Transferrin
10 Citations (Scopus)

Polyproline tetramer organizing peptides in fetal bovine serum acetylcholinesterase

Biberoglu, K., Schopfer, L. M., Saxena, A., Tacal, O. & Lockridge, O., Apr 1 2013, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1834, 4, p. 745-753 9 p.

Research output: Contribution to journalArticle

Acetylcholinesterase
Peptides
Serum
Butyrylcholinesterase
Amino Acids
16 Citations (Scopus)

Protein tyrosine adduct in humans self-poisoned by chlorpyrifos

Li, B., Eyer, P., Eddleston, M., Jiang, W., Schopfer, L. M. & Lockridge, O., Jun 15 2013, In : Toxicology and Applied Pharmacology. 269, 3, p. 215-225 11 p.

Research output: Contribution to journalArticle

Chlorpyrifos
Tyrosine
Proteins
Plasmas
Butyrylcholinesterase
1 Citation (Scopus)
Butyrylcholinesterase
Methylene Blue
methylene blue
Mass spectrometry
Mass Spectrometry
2012
13 Citations (Scopus)
Butyrylcholinesterase
Carbamates
carbamate (ester)
Poisons
protein
13 Citations (Scopus)
Histidine
Serum Albumin
Lysine
Tyrosine
Phosphates
18 Citations (Scopus)
Thiocholine
Carbofuran
Dichlorvos
Diazinon
Parathion
21 Citations (Scopus)

The proline-rich tetramerization peptides in equine serum butyrylcholinesterase

Biberoglu, K., Schopfer, L. M., Tacal, O. & Lockridge, O., Oct 1 2012, In : FEBS Journal. 279, 20, p. 3844-3858 15 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Proline
Horses
Peptides
Serum
2011
40 Citations (Scopus)

Biomarkers of organophosphorus (OP) exposures in humans

Marsillach, J., Richter, R. J., Kim, J. H., Stevens, R. C., MacCoss, M. J., Tomazela, D., Suzuki, S. M., Schopfer, L. M., Lockridge, O. & Furlong, C. E., Oct 1 2011, In : NeuroToxicology. 32, 5, p. 656-660 5 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Biomarkers
Aircraft
Serine
Tritolyl Phosphates
43 Citations (Scopus)

Exposure to tri-o-cresyl phosphate detected in jet airplane passengers

Liyasova, M., Li, B., Schopfer, L. M., Nachon, F., Masson, P., Furlong, C. E. & Lockridge, O., Nov 1 2011, In : Toxicology and Applied Pharmacology. 256, 3, p. 337-347 11 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Aircraft
Tritolyl Phosphates
Assays
Oils
27 Citations (Scopus)

Production of ES1 plasma carboxylesterase knockout mice for toxicity studies

Duysen, E. G., Koentgen, F., Williams, G. R., Timperley, C. M., Schopfer, L. M., Cerasoli, D. M. & Lockridge, O., Nov 21 2011, In : Chemical Research in Toxicology. 24, 11, p. 1891-1898 8 p.

Research output: Contribution to journalArticle

Carboxylesterase
Knockout Mice
Toxicity
Plasmas
Soman
39 Citations (Scopus)
Cholinesterases
Mass spectrometry
Mass Spectrometry
Phosphates
X-Rays
34 Citations (Scopus)

X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger

Nachon, F., Carletti, E., Wandhammer, M., Nicolet, Y., Schopfer, L. M., Masson, P. & Lockridge, O., Feb 15 2011, In : Biochemical Journal. 434, 1, p. 73-82 10 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Reaction intermediates
Fluorides
Anions
Catalytic Domain
2010
32 Citations (Scopus)

Detection of adduct on tyrosine 411 of albumin in humans poisoned by dichlorvos

Li, B., Ricordel, I., Schopfer, L. M., Baud, F., Mégarbane, B., Nachon, F., Masson, P. & Lockridge, O., Apr 15 2010, In : Toxicological Sciences. 116, 1, p. 23-31 9 p.

Research output: Contribution to journalArticle

Dichlorvos
Tyrosine
Albumins
Serum
Mass spectrometry
38 Citations (Scopus)

Development of diagnostics in the search for an explanation of aerotoxic syndrome

Schopfer, L. M., Furlong, C. E. & Lockridge, O., Sep 1 2010, In : Analytical Biochemistry. 404, 1, p. 64-74 11 p.

Research output: Contribution to journalArticle

Butyrylcholinesterase
Albumins
Covalent bonds
Poisons
Tritolyl Phosphates
25 Citations (Scopus)

Dichlorvos, chlorpyrifos oxon and Aldicarb adducts of butyrylcholinesterase, detected by mass spectrometry in human plasma following deliberate overdose

Li, B., Ricordel, I., Schopfer, L. M., Baud, F., Mégarbane, B., Massond, P. & Lockridgea, O., Aug 1 2010, In : Journal of Applied Toxicology. 30, 6, p. 559-565 7 p.

Research output: Contribution to journalArticle

Aldicarb
Dichlorvos
Plasma (human)
Butyrylcholinesterase
Pesticides
37 Citations (Scopus)
Organophosphates
Tyrosine
Peptides
Consensus Sequence
Ions
59 Citations (Scopus)
Chlorpyrifos
Tubulin
Microtubules
Brain
Microtubule Proteins