Janos Zempleni, PhD

Professor

  • 4106 Citations
  • 36 h-Index
1974 …2019
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Research Output 1989 2019

Filter
Article
2019
3 Citations (Scopus)
Dust
Macrophages
Milk
Nutrition
Polarization

Bovine milk extracellular vesicles (EVs) modification elicits skeletal muscle growth in rats

Parry, H. A., Brooks Mobley, C., Mumford, P. W., Romero, M. A., Haun, C. T., Zhang, Y., Roberson, P. A., Zempleni, J., Ferrando, A. A., Vechetti, I. J., McCarthy, J. J., Young, K. C., Roberts, M. D. & Kavazis, A. N., Jan 1 2019, In : Frontiers in Physiology. 10, MAR, 436.

Research output: Contribution to journalArticle

Open Access
Milk
Skeletal Muscle
Growth
Diet
Sonication
2018

A cell death assay for assessing the mitochondrial targeting of proteins

Camara Teixeira, D., Cordonier, E. L., Wijeratne, S. S. K., Huebbe, P., Jamin, A., Jarecke, S., Wiebe, M. S. & Zempleni, J., Jun 1 2018, In : Journal of Nutritional Biochemistry. 56, p. 48-54 7 p.

Research output: Contribution to journalArticle

Mitochondrial Proteins
Cell death
Assays
Cell Death
Mitochondrial Membranes
8 Citations (Scopus)
Exosome Multienzyme Ribonuclease Complex
Exosomes
Hand Strength
Nutrition
Inbred C57BL Mouse
1 Citation (Scopus)

Concentrations of purine metabolites are elevated in fluids from adults and infants and in livers from mice fed diets depleted of bovine milk exosomes and their RNA cargos

Aguilar-Lozano, A., Baier, S., Grove, R., Shu, J., Giraud, D., Leiferman, A., Mercer, K. E., Cui, J., Badger, T. M., Adamec, J., Andres, A. & Zempleni, J., Jan 1 2018, In : Journal of Nutrition. 148, 12, p. 1886-1894 9 p.

Research output: Contribution to journalArticle

Exosome Multienzyme Ribonuclease Complex
Milk
Diet
Liver
Urine
18 Citations (Scopus)

Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns

Manca, S., Upadhyaya, B., Mutai, E., Desaulniers, A. T., Cederberg, R. A., White, B. R. & Zempleni, J., Dec 1 2018, In : Scientific reports. 8, 1, 11321.

Research output: Contribution to journalArticle

Exosomes
Tissue Distribution
MicroRNAs
Milk
Transfer RNA
2017
41 Citations (Scopus)

Biological activities of extracellular vesicles and their cargos from bovine and human milk in humans and implications for infants

Zempleni, J., Aguilar-Lozano, A., Sadri, M., Sukreet, S., Manca, S., Wu, D., Zhou, F. & Mutai, E., Jan 1 2017, In : Journal of Nutrition. 147, 1, p. 3-10 8 p.

Research output: Contribution to journalArticle

Exosomes
Human Milk
Milk
MicroRNAs
Extracellular Vesicles
2016
8 Citations (Scopus)

Glucocorticoid cell priming enhances transfection outcomes in adult human mesenchymal stem cells

Kelly, A. M., Plautz, S. A., Zempleni, J. & Pannier, A. K., Feb 1 2016, In : Molecular Therapy. 24, 2, p. 331-341 11 p.

Research output: Contribution to journalArticle

Mesenchymal Stromal Cells
Glucocorticoids
Transfection
Transgenes
DNA
47 Citations (Scopus)

Human vascular endothelial cells transport foreign exosomes from cow’s milk by endocytosis

Kusuma, R. J., Manca, S., Frieme, T., Sukreet, S., Nguyen, C. & Zempleni, J., May 15 2016, In : American Journal of Physiology - Cell Physiology. 310, 10, p. C800-C807

Research output: Contribution to journalArticle

Exosomes
Endocytosis
Milk
Endothelial Cells
MicroRNAs
3 Citations (Scopus)

Inhibition of acetyl-CoA carboxylases by soraphen A prevents lipid accumulation and adipocyte differentiation in 3T3-L1 cells

Cordonier, E. L., Jarecke, S. K., Hollinger, F. E. & Zempleni, J., Jun 5 2016, In : European Journal of Pharmacology. 780, p. 202-208 7 p.

Research output: Contribution to journalArticle

3T3-L1 Cells
Acetyl-CoA Carboxylase
Adipocytes
Malonyl Coenzyme A
Fatty Acids
7 Citations (Scopus)

Riboflavin

Pinto, J. T. & Zempleni, J., Jan 1 2016, In : Advances in Nutrition. 7, 5, p. 973-975 3 p.

Research output: Contribution to journalArticle

2015
26 Citations (Scopus)

Computational characterization of exogenous microRNAs that can be transferred into human circulation

Shu, J., Chiang, K., Zempleni, J. & Cui, J., Nov 3 2015, In : PloS one. 10, 11, e0140587.

Research output: Contribution to journalArticle

MicroRNAs
microRNA
Milk
Lonicera
exosomes
17 Citations (Scopus)

Dietary microRNA database (DMD): An archive database and analytic tool for food-borne microRNAs

Chiang, K., Shu, J., Zempleni, J. & Cui, J., Jun 1 2015, In : PloS one. 10, 6, e0128089.

Research output: Contribution to journalArticle

MicroRNAs
microRNA
Databases
Food
Genes
36 Citations (Scopus)

Loss of miRNAs during processing and storage of cow's (Bos taurus) milk

Howard, K. M., Jati Kusuma, R., Baier, S. R., Friemel, T., Markham, L., Vanamala, J. & Zempleni, J., Jan 21 2015, In : Journal of Agricultural and Food Chemistry. 63, 2, p. 588-592 5 p.

Research output: Contribution to journalArticle

MicroRNAs
Milk
microwave treatment
microRNA
food processing

Resveratrol compounds inhibit human holocarboxylase synthetase and cause a lean phenotype in Drosophila melanogaster

Cordonier, E. L., Adjam, R., Teixeira, D. C., Onur, S., Zbasnik, R., Read, P. E., Döring, F., Schlegel, V. L. & Zempleni, J., Nov 1 2015, In : Journal of Nutritional Biochemistry. 26, 11, p. 1379-1384 6 p.

Research output: Contribution to journalArticle

Drosophila melanogaster
Phenotype
Vitis
Metabolism
Adipose Tissue
79 Citations (Scopus)
Exosomes
Caco-2 Cells
Endocytosis
Colon
Milk
2014
8 Citations (Scopus)
Histone Deacetylase 1
Co-Repressor Proteins
Terminal Repeat Sequences
Chromatin
Genes
14 Citations (Scopus)
Jurkat Cells
Riboflavin
Transcriptome
Lysine
Lymphoma
7 Citations (Scopus)
Biotinylation
Heat-Shock Proteins
Cell culture
Methionine
Lysine
161 Citations (Scopus)
HEK293 Cells
MicroRNAs
Blood Cells
Milk
Cell Culture Techniques
7 Citations (Scopus)

Novel roles of holocarboxylase synthetase in gene regulation and intermediary metabolism

Zempleni, J., Liu, D., Teixeira Camara, D. & Cordonier, E. L., Jan 1 2014, In : Nutrition Reviews. 72, 6, p. 369-376 8 p.

Research output: Contribution to journalArticle

Biotinylation
biotin carboxylase
HSP72 Heat-Shock Proteins
Genes
Gluconeogenesis
9 Citations (Scopus)

Off-target effects of sulforaphane include the derepression of long terminal repeats through histone acetylation events

Baier, S. R., Zbasnik, R., Schlegel, V. L. & Zempleni, J., Jan 1 2014, In : Journal of Nutritional Biochemistry. 25, 6, p. 665-668 4 p.

Research output: Contribution to journalArticle

Acetylation
Terminal Repeat Sequences
Histones
Genes
Brassica
4 Citations (Scopus)

Reply to witwer

Baier, S. R., Nguyen, C., Xie, F., Wood, J. R. & Zempleni, J., Jan 1 2014, In : Journal of Nutrition. 144, 11, 1 p.

Research output: Contribution to journalArticle

Reply to Witwer

Baier, S. R., Xie, F. & Zempleni, J., Nov 1 2014, In : The Journal of nutrition. 144, 11, p. 1882 1 p.

Research output: Contribution to journalArticle

Mononuclear Leukocytes
MicroRNAs
Milk
Gene Expression
5 Citations (Scopus)
Histone Code
Flavin-Adenine Dinucleotide
Monoamine Oxidase
Methylation
Lysine
7 Citations (Scopus)

β-Keto and β-hydroxyphosphonate analogs of biotin-5′-AMP are inhibitors of holocarboxylase synthetase

Sittiwong, W., Cordonier, E. L., Zempleni, J. & Dussault, P. H., Dec 15 2014, In : Bioorganic and Medicinal Chemistry Letters. 24, 24, p. 5568-5571 4 p.

Research output: Contribution to journalArticle

Adenosine Monophosphate
Inhibitory Concentration 50
Biotin
Histones
Molecules
2013
11 Citations (Scopus)

Biotinylation of lysine 16 in histone H4 contributes toward nucleosome condensation

Singh, M. P., Wijeratne, S. S. K. & Zempleni, J., Jan 15 2013, In : Archives of Biochemistry and Biophysics. 529, 2, p. 105-111 7 p.

Research output: Contribution to journalArticle

Biotinylation
Nucleosomes
Histones
Lysine
Condensation
1 Citation (Scopus)

Enrichment of meiotic recombination hotspot sequences by avidin capture technology

Camara Teixeira, D., Malkaram, S. A. & Zempleni, J., Mar 1 2013, In : Gene. 516, 1, p. 101-106 6 p.

Research output: Contribution to journalArticle

Avidin
Oligonucleotides
Genetic Recombination
Technology
DNA
7 Citations (Scopus)

Epigenetic Synergies Between Biotin and Folate in the Regulation of Pro-Inflammatory Cytokines and Repeats

Xue, J. & Zempleni, J., Nov 1 2013, In : Scandinavian Journal of Immunology. 78, 5, p. 419-425 7 p.

Research output: Contribution to journalArticle

Biotin
Folic Acid
Epigenomics
Cytokines
Terminal Repeat Sequences
6 Citations (Scopus)
HSP72 Heat-Shock Proteins
Biotinylation
HEK293 Cells
Mass Spectrometry
Biotin
14 Citations (Scopus)
Histone-Lysine N-Methyltransferase
Biotinylation
Methylation
Histones
Histone Code
13 Citations (Scopus)

Holocarboxylase synthetase synergizes with methyl CpG binding protein 2 and DNA methyltransferase 1 in the transcriptional repression of long-terminal repeats

Xue, J., Wijeratne, S. S. K. & Zempleni, J., Jan 1 2013, In : Epigenetics. 8, 5, p. 504-511 8 p.

Research output: Contribution to journalArticle

Methyl-CpG-Binding Protein 2
Terminal Repeat Sequences
Methyltransferases
DNA
Chromatin
14 Citations (Scopus)

Identification and assessment of markers of biotin status in healthy adults

Eng, W. K., Giraud, D., Schlegel, V. L., Wang, D., Lee, B. H. & Zempleni, J., Jul 28 2013, In : British Journal of Nutrition. 110, 2, p. 321-329 9 p.

Research output: Contribution to journalArticle

Biotin
Methylmalonyl-CoA Decarboxylase
biotin carboxylase
methylcrotonoyl-CoA carboxylase
Lymphocytes
3 Citations (Scopus)

Three promoters regulate the transcriptional activity of the human holocarboxylase synthetase gene

Xia, M., Malkaram, S. A. & Zempleni, J., Nov 1 2013, In : Journal of Nutritional Biochemistry. 24, 11, p. 1963-1969 7 p.

Research output: Contribution to journalArticle

Human Activities
Genes
Biotin
Cells
Cell Line
2012
8 Citations (Scopus)
Biotinylation
Methylation
Cytosine
Histones
Genes
7 Citations (Scopus)

Effects of single-nucleotide polymorphisms in the human holocarboxylase synthetase gene on enzyme catalysis

Esaki, S., Malkaram, S. A. & Zempleni, J., Apr 1 2012, In : European Journal of Human Genetics. 20, 4, p. 428-433 6 p.

Research output: Contribution to journalArticle

Catalysis
Single Nucleotide Polymorphism
Biotin
Enzymes
Genes
9 Citations (Scopus)
Jurkat Cells
Biotin
Histones
Biotinylation
Genes
2011
3 Citations (Scopus)

A 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity

Rios-Avila, L., Prince, S. A., Wijeratne, S. S. K. & Zempleni, J., Apr 11 2011, In : Clinica Chimica Acta. 412, 9-10, p. 735-739 5 p.

Research output: Contribution to journalArticle

Assays
Throughput
Biotinylation
Histones
Holocarboxylase Synthetase Deficiency
41 Citations (Scopus)

Biotinylation is a natural, albeit rare, modification of human histones

Kuroishi, T., Rios-Avila, L., Pestinger, V., Wijeratne, S. S. K. & Zempleni, J., Dec 1 2011, In : Molecular Genetics and Metabolism. 104, 4, p. 537-545 9 p.

Research output: Contribution to journalArticle

Histone Code
Biotinylation
Histones
Chromatin
Multiprotein Complexes
1 Citation (Scopus)

Development of an internet based system for modeling biotin metabolism using Bayesian networks

Zhou, J., Wang, D., Schlegel, V. L. & Zempleni, J., Nov 1 2011, In : Computer Methods and Programs in Biomedicine. 104, 2, p. 254-259 6 p.

Research output: Contribution to journalArticle

Bayesian networks
Biotin
Metabolism
Internet
Metabolites
25 Citations (Scopus)

Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18

Bao, B., Pestinger, V., Hassan, Y. I., Borgstahl, G. E., Kolar, C. & Zempleni, J., May 1 2011, In : Journal of Nutritional Biochemistry. 22, 5, p. 470-475 6 p.

Research output: Contribution to journalArticle

Biotinylation
Histones
Chromatin
Biotin
Proteins
10 Citations (Scopus)

Human holocarboxylase synthetase with a start site at methionine-58 is the predominant nuclear variant of this protein and has catalytic activity

Bao, B., Wijeratne, S. S. K., Rodriguez-Melendez, R. & Zempleni, J., Aug 19 2011, In : Biochemical and Biophysical Research Communications. 412, 1, p. 115-120 6 p.

Research output: Contribution to journalArticle

Nuclear Proteins
Methionine
Catalyst activity
Proteins
Cell Nucleus
9 Citations (Scopus)
Methyltransferases
Adenine
Chromatin
Breast
Binding Sites
37 Citations (Scopus)

Novel histone biotinylation marks are enriched in repeat regions and participate in repression of transcriptionally competent genes

Pestinger, V., Wijeratne, S. S. K., Rodriguez-Melendez, R. & Zempleni, J., Apr 1 2011, In : Journal of Nutritional Biochemistry. 22, 4, p. 328-333 6 p.

Research output: Contribution to journalArticle

Histone Code
Biotinylation
Histones
Genes
Chromatin
24 Citations (Scopus)

The role of histone H4 biotinylation in the structure of nucleosomes

Filenko, N. A., Kolar, C., West, J. T., Abbie Smith, S., Hassan, Y. I., Borgstahl, G. E., Zempleni, J. & Lyubchenko, Y. L., Feb 7 2011, In : PloS one. 6, 1, e16299.

Research output: Contribution to journalArticle

biotinylation
Biotinylation
nucleosomes
Nucleosomes
histones
5 Citations (Scopus)

The role of holocarboxylase synthetase in genome stability is mediated partly by epigenomic synergies between methylation and biotinylation events

Zempleni, J., Li, Y., Xue, J. & Cordonier, E. L., Jan 1 2011, In : Epigenetics. 6, 7, p. 892-894 3 p.

Research output: Contribution to journalArticle

Biotinylation
Genomic Instability
Epigenomics
Histones
Methylation
2010
20 Citations (Scopus)

Biotin regulates the expression of holocarboxylase synthetase in the miR-539 pathway in HEK-293 cells

Bao, B., Rodriguez-Melendez, R., Wijeratne, S. S. K. & Zempleni, J., Sep 1 2010, In : Journal of Nutrition. 140, 9, p. 1546-1551 6 p.

Research output: Contribution to journalArticle

HEK293 Cells
Biotin
MicroRNAs
biotin carboxylase
Kidney
17 Citations (Scopus)

Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver cells

Mall, G. K., Chew, Y. C. & Zempleni, J., Jun 1 2010, In : Journal of Nutrition. 140, 6, p. 1086-1092 7 p.

Research output: Contribution to journalArticle

Jurkat Cells
Hep G2 Cells
Biotin
Lymphocytes
Liver
21 Citations (Scopus)

K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts

Wijeratne, S. S. K., Camporeale, G. & Zempleni, J., Apr 1 2010, In : Journal of Nutritional Biochemistry. 21, 4, p. 310-316 7 p.

Research output: Contribution to journalArticle

Telomere
Fibroblasts
Histones
Biotinylation
Lung
5 Citations (Scopus)
Biotinylation
Peptides
Lysine
Adenosine Monophosphate
Histones